Hello,I tried this (with some modifications: Other media, using...

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Markus Oelze
Mar 26, 2013
protocol Protocol: Soft–Agar colony Formation Assay
Hello,

I tried this (with some modifications: Other media, using a 12-well plate and so on). I read somewhere to put some Media on top an change it every 3 days. At first it works just fine, colonies are forming. But after approximately 5 days, the colonies just die. Do you have
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FengZhi Liu Author Answered Apr 9, 2013

School of Biomedical Sciences, Thomas Jefferson University, USA

Hi I had not done this way to keep colonies. But I guess the ratio of colony size to media may be the cause. This is similar to keep cells in a flask. You either threw away some cells or decrease the serum concentration in the media.
It is the same to bacteria growth curve.
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