Improve Research Reproducibility A Bio-protocol resource
- Home
- Protocols
-
Hello,It is very interesting method; I have two questions;1....
IG
Itshak Golan Mar 21, 2013
Hello,
It is very interesting method; I have two questions;
1. What are the weaknesses of this method? In which conditions it is not good to use it?
2. Do you have experiance with anti-CD44 antibodies?
Thank you in advance for your replay.
Best Regards,
Dr. I. Read more
It is very interesting method; I have two questions;
1. What are the weaknesses of this method? In which conditions it is not good to use it?
2. Do you have experiance with anti-CD44 antibodies?
Thank you in advance for your replay.
Best Regards,
Dr. I. Read more
3
Views
Answer
Recommend
Follow
Share
2
answers
Sort by: most helpful / most recent
Itshak Golan Answered Mar 21, 2013
Swansea University
Thank you
0 helpful
0 unhelpful
0 comments Share
Zheng Liu Author Answered Mar 21, 2013
Bio-protocol
I am glad that you thought it was interesting. As to you questions,
1. whole blood staining does not work with all antibodies or cell subsets. For instance, we tried and failed to stain human DC subsets with whole blood staining using a 9 antibody mix. So pilot experiments are needed to determine if your antibodies are compatible with this method.
2. I have no experience with anti-CD44 antibodies.
Hope my answers are helpful.
Regards,
Dr. Z. Liu
1. whole blood staining does not work with all antibodies or cell subsets. For instance, we tried and failed to stain human DC subsets with whole blood staining using a 9 antibody mix. So pilot experiments are needed to determine if your antibodies are compatible with this method.
2. I have no experience with anti-CD44 antibodies.
Hope my answers are helpful.
Regards,
Dr. Z. Liu
0 helpful
0 unhelpful
0 comments ShareMy answer
Write your answer...
References (optional)
Add more
Post an Answer