Is it possible to do this experiment without Triton X-100?

Feb 25, 2013
protocol Protocol: Propidium Iodide Staining of Cells for FACS Analysis
Is it possible to do this experiment without Triton X-100?
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Roberta Ruela Answered Sep 4, 2013

Unicamp

You can also use a hypotonic buffer instead of Triton X-100 to permeabilize the cells.
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Hui Zhu Author Answered Feb 27, 2013

Stanford University

Triton X-100 is used as permeabilization buffer to penetrate cells. If you don’t penetrate cells, Propidium Iodide won’t stain well.
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