Is glutamine an essential component in the glucose production...

Feb 2, 2013
protocol Protocol: Glucose Production Assay in Primary Mouse Hepatocytes
Is glutamine an essential component in the glucose production buffer? And how critical is the pH 7.4 in the media? Is there any impact on glucose production if the pH is 8.0?
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Michihiro Matsumoto Author Answered Feb 6, 2013

Department of Molecular Metabolic Regulation, Diabetes Research Center, Research Institute, National Center for Global Health and Medicine, Japan

We think that glutamine is not essential, but we never perform the experiment to prove it. I don't know the impact of pH 8.0 on glucose production.
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