Improve Research Reproducibility A Bio-protocol resource

here u include std PCR Protocol but i come to know d annealing...

 Jan 22, 2013
protocol Protocol: Standard PCR Protocol
here u include std PCR Protocol but i come to know d annealing temperature for alkaline phosphatase PCR please
mention my query and solve it....THANK YOU
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Fanglian He Author Answered Jan 23, 2013

Bio-protocol

I am not sure I understand your question well. To my knowledge, alkaline phosphatase is used to clean up PCR product by removing excess dNTPs (although I never used alkaline phosphatase for this purpose). So, I assume this step is done after PCR reaction.
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