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Hi I wanted to ask if its possible to use gel retardation on...

 Jan 8, 2013
protocol Protocol: A General EMSA (Gel-shift) Protocol
Hi I wanted to ask if its possible to use gel retardation on Sigma B as i believe it is the regulator of my target gene in S.aureus, or if there is a better protocol or method?

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Visitor Answered Jan 10, 2013

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Gel retardation is a good option if you know the candidate binding sites in the promoter region of your target gene. In your case, my opinion is that Ch-IP could be better choice.
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