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This is really helpful to me. I'm wondering if you have experience...

 Jan 7, 2013
protocol Protocol: Whole Blood Staining of Human Monocyte Subsets for Flow Cytometry
This is really helpful to me. I'm wondering if you have experience with CD11b and ly-6c in whole mouse blood. I have noticed that samples from whole mouse blood usually show incomplete lysis of red blood cells, which result in a difficulty in identify monocytes in FSC-SSC picture. Have yo Read more Read less more less
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Zheng Liu Author Answered Jan 19, 2013

Bio-protocol

No, I have no experience with CD11b and Ly6C in mouse whole blood. As to RBC lysis, I recommend BD’s PharmLysis which usually results in a pretty complete lysis. But I only used it before staining the cells with antibodies.
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