The blade has a certain thickness, can be directly observed...

Dec 9, 2012
protocol Protocol: Detection of Hydrogen Peroxide by DAB Staining in Arabidopsis Leaves
The blade has a certain thickness, can be directly observed with a microscope.how to photographs on a plain white background under uniform lighting.Thank you!
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Arsalan Daudi Author Answered Dec 22, 2012

University of California

Depends on what you want to look at. If you want an overview of the distribution and overall intensity of the DAB stain, then just place the de-stained leaf on a plain white background under uniform lighting and you should get a clear image of the stain.

If you want to look at it under the microscope, just mount the leaf as you would normally on a glass slide and view under a light microscope. As the stain requires no phase contrast or fluorescence detection, it is simple to adjust the plane according to what cells you wish to see.
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