I noticed several salt crystals post SA-bGal staining, which...
I noticed several salt crystals post SA-bGal staining, which do not seem to dissolve after washing. These crystals interfere during counting and also while taking pictures. Is there anyway to troubleshoot this problem?
Thanks,
Vijay
3
answers
Sort by: most helpful / most recent
yan huang Answered Dec 5, 2019
Sun Yat-sen university
I tried to use ethanol to remove the crystals and it works, 3-5 hours enough, hope to help you.
My first question is about what type pf water should i use to remove the crystals?
The number of my crystals is so many and attached to the flask surface that i can't read the results properly. so how do I dissolve them
my second question is about false positive staining
I have observed blue stained cells in control sample of young , cells weren't over confluent , is it normal to have some blue staining in control young cells
my cells were Periodontal Ligament fibroblasts, and they were stained two starting from passage 2
Thanks a lot
Nada
Michael Eccles Author Answered Nov 28, 2012
Department of Pathology, University of Otago, New Zealand
We have also seen these crystals before. However, we have always managed to take images without excessive interference from the crystals. Our only suggestion is to do as many washes with water after the staining as necessary.
References (optional)
Add more
Post an Answer