I noticed several salt crystals post SA-bGal staining, which...

Nov 26, 2012
protocol Protocol: Senescence Associated β-galactosidase Staining
I noticed several salt crystals post SA-bGal staining, which do not seem to dissolve after washing. These crystals interfere during counting and also while taking pictures. Is there anyway to troubleshoot this problem?

Thanks,
Vijay
answer Answer
recommend recommend recommend recommend Recommend
follow follow follow follow Follow
share share Share
3 answers
Sort by: most helpful / most recent

yan huang Answered Dec 5, 2019

Sun Yat-sen university

I tried to use ethanol to remove the crystals and it works, 3-5 hours enough, hope to help you.
0 helpful
0 unhelpful
comment 0 comments down up
share Share

Nada Alshehri Answered Oct 13, 2015

Forsyth institute

My first question is about what type pf water should i use to remove the crystals?

The number of my crystals is so many and attached to the flask surface that i can't read the results properly. so how do I dissolve them


my second question is about false positive staining
I have observed blue stained cells in control sample of young , cells weren't over confluent , is it normal to have some blue staining in control young cells
my cells were Periodontal Ligament fibroblasts, and they were stained two starting from passage 2
Thanks a lot
Nada
0 helpful
0 unhelpful
comment 0 comments down up
share Share

Michael Eccles Author Answered Nov 28, 2012

Department of Pathology, University of Otago, New Zealand

We have also seen these crystals before. However, we have always managed to take images without excessive interference from the crystals. Our only suggestion is to do as many washes with water after the staining as necessary.
0 helpful
0 unhelpful
comment 0 comments down up
share Share

My answer

Write your answer...

References (optional)

add Add more

post Post an Answer