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请问E3是什么试剂？可以保证胚胎不悬浮不脱水。
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景 枫 Oct 17, 2012
请问E3是什么试剂？可以保证胚胎不悬浮不脱水。
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Lili Jing Author Answered Oct 25, 2012
Department of Cell and Molecular Biology, University of Pennsylvania, USA
E3 是斑马鱼胚胎培养液。可以让其不脱水和保持活性。做显微注射的时候不要加太多E3，胚胎就不会悬浮。
配方见下。
60X E3 stock
(makes 2 liters)
34.4 g NaCl
1.52 g KCl
5.8 g CaCl2.2H2O
9.8 g MgSO4.7H2O
add double distilled water up to 2000 ml
Store in refrigerator. To dilute to 1X for rearing zebrafish, use 160 ml of stock and fill to 10 liters with ddH2O.
配方见下。
60X E3 stock
(makes 2 liters)
34.4 g NaCl
1.52 g KCl
5.8 g CaCl2.2H2O
9.8 g MgSO4.7H2O
add double distilled water up to 2000 ml
Store in refrigerator. To dilute to 1X for rearing zebrafish, use 160 ml of stock and fill to 10 liters with ddH2O.
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