Improve Research Reproducibility A Bio-protocol resource

I am using your protocol to do phosphate assays, and I am curious:...

 Oct 15, 2012
protocol Protocol: Arabidopsis Hydroponics and Shoot Branching Assay
I am using your protocol to do phosphate assays, and I am curious: do you make your Hoagland's in lab or do you purchase it? If you purchase it, from where? Thank you.
answer Answer
recommend recommend recommend recommend Recommend
follow follow follow follow Follow
share share Share
4 answers
Sort by: most helpful / most recent

Mark T Waters Author Answered Feb 12, 2013

ARC Centre of Excellence in Plant Energy Biology, The University of Western Australia, Australia

And more information on what rock wool actually is:

http://www.grodan.com/about+grodan/stone+wool+substrate
0 helpful
0 unhelpful
comment 0 comments down up
share Share

Mark T Waters Author Answered Feb 12, 2013

ARC Centre of Excellence in Plant Energy Biology, The University of Western Australia, Australia

Hi Charles

We get our rock wool from these guys in Australia:

http://www.growroom.com.au/shop/propagation/rockwool-wrapped-slab-75x900x150/

The manufacturer is Grodan, and you may be able to find alocal distributor here:

http://www.grodan.com/about+grodan/find+a+distributor
0 helpful
0 unhelpful
comment 0 comments down up
share Share

Charles Parrish Answered Feb 5, 2013

North Carolina State University

Thank you, Dr. Waters. I would also like to know where you purchased the rockwool used in your experiment, and what type of rockwool it is? Thank you.
0 helpful
0 unhelpful
comment 0 comments down up
share Share

Mark T Waters Author Answered Oct 15, 2012

ARC Centre of Excellence in Plant Energy Biology, The University of Western Australia, Australia

We make each of the stock solutions ourselves in the lab. This is potentially expensive if you have to buy all the reagents, especially considering how little you need of some of the micronutrients. Some of the micronutrients can be substituted, e.g. ZnCl2 rather than ZnSO4, as it is the Zn2+ rather than the Cl- or SO4- that matters.
0 helpful
0 unhelpful
comment 0 comments down up
share Share

My answer

Write your answer...

References (optional)

add Add more

post Post an Answer