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why do you maintain cells in gelatin coated dishes for...

 Oct 1, 2012
protocol Protocol: Culture, Differentiation and Transfection of C2C12 Myoblasts
why do you maintain cells in gelatin coated dishes for differentiation? Are the plastic wares used for culturing c2c12 cells different from the ones that are used for other cell lines say 293-T? I have been using 293-T cells and I am switching over to c2c12 cells for some assays. I would l Read more Read less more less
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Lili Jing Author Answered Oct 3, 2012

Department of Cell and Molecular Biology, University of Pennsylvania, USA

Gelatin coated plates promote the cell attachment and spreading of myoblasts. C2C12 grow fine on regular plastic plates, but the easy grip plate is better.

The information of these plates can be found:

http://www.thermoscientific.com/ecomm/servlet/productsdetail_11152___12976170_-1

http://www.bdbiosciences.com/ecat/Searchresults.do?pgNum=1&pgSize=&sort=SortOrderDef&check=mainsearchcheck&key=gelatin+plates&mterms=true
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