How many passages can these cells hold on to. i.e suppose I...

Oct 1, 2012
protocol Protocol: Culture, Differentiation and Transfection of C2C12 Myoblasts
How many passages can these cells hold on to. i.e suppose I have cells of passage no.1, how long can it go before i thaw a fresh vial?

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Sowmya L
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Lili Jing Author Answered Oct 3, 2012

Department of Cell and Molecular Biology, University of Pennsylvania, USA

The cells can be used up to passages 35-40. We haven't seen a big difference between the low passage and the high passage.
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