why is it green in color is it due to the reaction of of an...

Aug 9, 2012
protocol Protocol: Pollen Fertility/viability Assay Using FDA Staining
why is it green in color is it due to the reaction of of an enzyme esterase?
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Xiyan Li Author Answered Nov 17, 2012

Department of Genetics, Stanford University, USA

Yes. FDA fluoresces only after being hydrolyzed by a cytosolic esterase.This enzyme is leaking out in dead pollen grains.
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