Hi,What do you consider "differentiation under starvation media"....

Jul 12, 2012
protocol Protocol: Culture, Differentiation and Transfection of C2C12 Myoblasts
Hi,
What do you consider "differentiation under starvation media". Is ist DMEM with 1%FBS or DEMEM without any serum?
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Becky Diebold Answered Oct 1, 2013

Emory University

Have you tried Xtreme Gene HP to transfect these cells? Roche has replaced Fugene products with XtremeGene products. I was wondering if Xtreme Gene HP works the same as Fugene HD? Roche recommends 70-90% confluency before transfecting with XtremeGene HP, but maybe 50% would be better? If you have experience with this, please reply. Thank you.
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Lili Jing Author Answered Jul 16, 2012

Department of Cell and Molecular Biology, University of Pennsylvania, USA

Either DMEM with 1% horse serum or without any serum will work. But myotubes seem to be more readily formed in medium containing 1% serum than in serum-free condition.
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