Have you tried Xtreme Gene HP to transfect these cells? Roche has replaced Fugene products with XtremeGene products. I was wondering if Xtreme Gene HP works the same as Fugene HD? Roche recommends 70-90% confluency before transfecting with XtremeGene HP, but maybe 50% would be better? If you have experience with this, please reply. Thank you.
Department of Cell and Molecular Biology, University of Pennsylvania, USA
Either DMEM with 1% horse serum or without any serum will work. But myotubes seem to be more readily formed in medium containing 1% serum than in serum-free condition.