I just want to ask if what is the normal fasting blood glucose...

Jul 7, 2012
protocol Protocol: Glucose Tolerance Test in Mice
I just want to ask if what is the normal fasting blood glucose level of mice fasted for 12 hours? Thank you.
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Peichuan Zhang Author Answered Jul 8, 2012

Acepodia Biotech

Hello,

We used both B6 black mice as well as littermates (sibling) of the PERK knockout mice as the controls. The fasting plasma glucose level was around 100 mg/dL (~5.5 mmol) or maybe even lower. I recall that the technician moved the mice into a FRESH cage with water just before he left (around 6PM), and then we perform the test next morning or around noon (~16 to 18 hours).

The PERK knockout mice had mixed genetic background of B6 and 129. Please be aware that the fasting levels might be different for mice from different backgrouns --- refer to http://ajpendo.physiology.org/content/295/6/E1323.full Andrikopoulos et al, Am J Physiol Endocrinol Metab, 2008.

Please feel free to ask us questions

Thanks,
Peichuan
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