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This is excellent. I'm wondering if you have experience with...

 Jun 9, 2012
protocol Protocol: Whole Blood Staining of Human Monocyte Subsets for Flow Cytometry
This is excellent. I'm wondering if you have experience with CD86. I've noticed others use CD86 to get all monocytes, then identify the subsets with CD14/16. Have you tried this? Thanks.
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Zheng Liu Author Answered Jan 19, 2013

Bio-protocol

As far as I know, CD86 is an activation marker which is not limited to monocytes. Therefore I personally don’t think you can get all monocytes using CD86.
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