I have problems to detect the florescence of PI and GFP together,...

Jun 8, 2012
protocol Protocol: Cell Cycle Analysis Using Propidium Iodide Staining with GFP Detection
I have problems to detect the florescence of PI and GFP together, as their emission is very similar. Any suggestions?
Thanks.
answer Answer
recommend recommend recommend recommend Recommend
follow follow follow follow Follow
share share Share
1 answer
Sort by: most helpful / most recent

hui zhu Answered Jun 10, 2012

stanford universtiy

PI is much more broader which fluoresces everywhere from yellow to far-red.
You need to do compensation when go with GFP. You can use 7-AAD in place of
PI, which is more tighter.
0 helpful
0 unhelpful
comment 0 comments down up
share Share

My answer

Write your answer...

References (optional)

add Add more

post Post an Answer