Improve Research Reproducibility A Bio-protocol resource
- Home
- Protocols
-
Hi,I would like to make this assay (modifying something) and...
CT
Carmen Toro May 8, 2012
Hi,
I would like to make this assay (modifying something) and then trypsinize the cells in order to obtain single cells to do patch clamp on those cells. Is that possible? How can I to obtain two groups: migrating and no migrating cells? Could you please give me some suggestion?
Read more
I would like to make this assay (modifying something) and then trypsinize the cells in order to obtain single cells to do patch clamp on those cells. Is that possible? How can I to obtain two groups: migrating and no migrating cells? Could you please give me some suggestion?
Read more
2
Views
Answer
Recommend
Follow
Share
1
answer
Sort by: most helpful / most recent
Yanling Chen Author Answered Jun 1, 2012
The Scripps Research Institute
For the patch clamp assay, you probably have to try it because I do not have this information.
For seperating/obtaining migrating and non-migrating cells (again sorry I don't have this info). You might want to try a different method, e.g. cell invasion assay using matrigel and then retrieve the migrated cells from the gel? Just my suggestion.
For seperating/obtaining migrating and non-migrating cells (again sorry I don't have this info). You might want to try a different method, e.g. cell invasion assay using matrigel and then retrieve the migrated cells from the gel? Just my suggestion.
0 helpful
0 unhelpful
0 comments ShareMy answer
Write your answer...
References (optional)
Add more
Post an Answer