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How many CD4+CD44loCD62Lhi T cells could you sort from one mouse...

 May 2, 2012
protocol Protocol: In vitro Differentiation of Mouse Th0, Th1 and Th2 from Naïve CD4 T Cells
How many CD4+CD44loCD62Lhi T cells could you sort from one mouse (WT)? How many wells did you use for each condition (Th0/Th1/Th2/Th17)? Many thanks.
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Jia Li Author Answered Jul 9, 2013

SRI International

about several million naive cells can be obtained from WT. Usually at least three wells for each condition so that the results can do statistic analysis.
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