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Good evening Sir/Mam,I, G.Vinod kumar, working as a research...
Good evening Sir/Mam,
I, G.Vinod kumar, working as a research scholar in Department of zoology India. In my resarch work i am going to concentrate on some biochemical aspects i.e., total protein (µg/mg), total glycogen (µg/mg), total carbohydrate (µg/mg), total DNA (µg/mg), tot Read more
I, G.Vinod kumar, working as a research scholar in Department of zoology India. In my resarch work i am going to concentrate on some biochemical aspects i.e., total protein (µg/mg), total glycogen (µg/mg), total carbohydrate (µg/mg), total DNA (µg/mg), tot Read more
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Peichuan Zhang Answered Apr 18, 2012
Calico Life Sciences
Hi,
I have only used the above protocol to measure glycogen concentrations in the liver. I'm not quite that I fully understand your question though. From the description of your experiments, I believe the only way is to measure each single parameter, using respective methods/kits, and then normalize the quantities to the weight of the dry tissue.
TRIZol (Invitrogen) is supposed to help the isolation of DNA/RNA/protein from just one prep. However, I don't think that the isolate quality is good enough. Otherwise, I'd say that you may consider the most common methods to measure these
Protein - Bradford assay or BCA assay
Carbohydrate - Phenol/sulfuric acid method
DNA/RNA - Tissue extraction kit (Qiagen)
Amino acids - Chromatography
GST - ELISA kit
I don't think that we have all the protocols available here at our portal, and we will try to collect all these protocols.
Best,
Peichuan
I have only used the above protocol to measure glycogen concentrations in the liver. I'm not quite that I fully understand your question though. From the description of your experiments, I believe the only way is to measure each single parameter, using respective methods/kits, and then normalize the quantities to the weight of the dry tissue.
TRIZol (Invitrogen) is supposed to help the isolation of DNA/RNA/protein from just one prep. However, I don't think that the isolate quality is good enough. Otherwise, I'd say that you may consider the most common methods to measure these
Protein - Bradford assay or BCA assay
Carbohydrate - Phenol/sulfuric acid method
DNA/RNA - Tissue extraction kit (Qiagen)
Amino acids - Chromatography
GST - ELISA kit
I don't think that we have all the protocols available here at our portal, and we will try to collect all these protocols.
Best,
Peichuan
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