Improve Research Reproducibility A Bio-protocol resource

Hi,thanks for your sharing! i really want to know more details...

 Apr 11, 2012
protocol Protocol: Isolation and Culture of Mouse Bone Marrow-derived Macrophages (BMM’phi’)
Hi,thanks for your sharing!
i really want to know more details about the procedure if i use the M-CSF to the culture of BMDM,such as the exact concentration of M-CSF, when to change the cutlure and so on.
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Ran Chen Author Answered Apr 14, 2012

Zhejiang JFK Biological Technology Co. Ltd.

Use 20 ng/ml M-CSF instead of L-conditioned medium.
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