Is it M-CSF or GM-CSF necessary for the microglia culture?

Apr 6, 2012
protocol Protocol: Generation of Microglia Cultures and Mixed Glial Culture
Is it M-CSF or GM-CSF necessary for the microglia culture?
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Huiming Gao Author Answered Apr 7, 2012

Neuropharmacology Section, National Institute of Environmental Health Sciences, National Institutes of Health, Research Triangle Park, USA

M-CSF and GM-CSF have been found to induce microglial proliferation in fetal and adult cultures. However, we have never added exogenous M-CSF or GM-CSF into our culture medium. It is possible that low levels of M-CSF and/or GM-CSF in fetal bovine serum may help microglia cultures grow.
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