Neuropharmacology Section, National Institute of Environmental Health Sciences, National Institutes of Health, Research Triangle Park, USA
M-CSF and GM-CSF have been found to induce microglial proliferation in fetal and adult cultures. However, we have never added exogenous M-CSF or GM-CSF into our culture medium. It is possible that low levels of M-CSF and/or GM-CSF in fetal bovine serum may help microglia cultures grow.