why there is need to stain with crystal violet? can we visualize...
why there is need to stain with crystal violet? can we visualize the cells inside the wells or we have to take out the round coverslip?
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i wanna ask..after staining with crystal violet solution...is there need to remove the staining solution before taking photo?ASAP
Yanling Chen Author Answered Jun 1, 2012
The Scripps Research Institute
Staining with crystal violet (other other dyes of your choice) would make visualization easier; also better for taking pictures.
And if you need to grow cells on coverslips (that may be coated with reagents of your interest), you will need to modify/optimize the protocols.
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