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After you transfer the culture to a new plate, do you still...

 Mar 1, 2012
protocol Protocol: In vitro Differentiation of Mouse Th0, Th1 and Th2 from Naïve CD4 T Cells
After you transfer the culture to a new plate, do you still need to have anti-CD3 and antiCD28?
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Jia Li Author Answered Mar 2, 2012

SRI International

No need to add anti-CD3 and anti-CD28. Because anti-CD3 will cause cell death, that's why anti-CD3 is plate-bound and we transfer culture to new plate.
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