how can i increase the titre of the virus.Will the helper plasmids...

Feb 27, 2012
protocol Protocol: Lentivirus Production
how can i increase the titre of the virus.Will the helper plasmids ratio alteration help in boosting virus production.
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Nabila Aboulaich Author Answered Feb 29, 2012

This is a link to a paper where they study the factors affecting
lentivirus titer.
http://www.aaronlogan.com/downloads/Logan_infectivity.pdf
Clontech has a kit for lentivirus concentration that would increase the titer by 100 fold. Also ultracentrifugation can be used to concentarte lentivirus.

I hope this answers the question.
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