We wish to know whether there is an universal equation for calculating TG concentration in the liver (Tg (mg)/Liver (gm) from the CTE (mg/dL) value obtained in a TG assay? Could you explain to us your equation: CTE (mg/dL)*(10/30)*(415/200)*0.012(dL)/wt(gr)that is listed in the protocol "MRead moreRead less
Division of Endocrinology, Gerontology and Metabolism, Stanford University School of Medicine, USA
There is no universal equation for calculating TG concentration. You can check protocols of commercially available TG kits and you will see several methods/equation for calculation.
In the equation, the (10/30) is the ratio of standard used (10ul) to the total volume of test sample which is 30ul. The (415/200): the 41corresponds to the volume (415ul) from where the tested sample came out (step 10: Move 200 µl to new epp tube, add 215 µl 1M MgCl2, vortex) The 200 is for the 200ul that we took out of step 9 Finally the 0.012 corresponds to the the final volume obtained in step 9 (1200ul) Hope this helps