Improve Research Reproducibility A Bio-protocol resource

Will MDA-MB-231 cells migrate/invade with FBS and not EGF? I...

 Jan 24, 2012
protocol Protocol: Transwell Cell Migration Assay Using Human Breast Epithelial Cancer Cell
Will MDA-MB-231 cells migrate/invade with FBS and not EGF? I have read several papers that imply this cell line will not invade/migrate without it. Thanks!
answer Answer
recommend recommend recommend recommend Recommend
follow follow follow follow Follow
share share Share
1 answer
Sort by: most helpful / most recent

Yanling Chen Author Answered Jan 28, 2012

The Scripps Research Institute

In the author's hands, EGF has never been required for successful migration assay using MB231 cells. Some researchers even carry out this assay using medium without either FBS or EGF. Please see reference: PLoS One. 2010 Dec 30;5(12):e15940.
0 helpful
0 unhelpful
comment 0 comments down up
share Share

My answer

Write your answer...

References (optional)

add Add more

post Post an Answer