Neuropharmacology Section, National Institute of Environmental Health Sciences, National Institutes of Health, Research Triangle Park, USA
If you use transwell inserts to generate reconstituted cultures that contain neurons and glia, you can separate neurons from glia. Otherwise, it is impossible to separate neurons from glia in the mixed neuron-glia cultures. If you consider using transwells, I can upload my protocol for generating that type of reconstituted cultures.