does anybody knows if i can separate neurones from mixed...

protocol Protocol: Midbrain Neuron-glia Mixed Cultures
does anybody knows if i can separate neurones from mixed cultures?
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Huiming Gao Author Answered Jan 6, 2012

Neuropharmacology Section, National Institute of Environmental Health Sciences, National Institutes of Health, Research Triangle Park, USA

If you use transwell inserts to generate reconstituted cultures that contain neurons and glia, you can separate neurons from glia. Otherwise, it is impossible to separate neurons from glia in the mixed neuron-glia cultures. If you consider using transwells, I can upload my protocol for generating that type of reconstituted cultures.
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