Dear Xiyan:您好,请问GUS基因在体外表达,怎么检测呢,有这方面的protocol吗?谢谢!

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youtao chen
Sep 14, 2011
protocol Protocol: Histostaining for Tissue Expression Pattern of Promoter-driven GUS Activity in Arabidopsis
Dear Xiyan:

您好,请问GUS基因在体外表达,怎么检测呢,有这方面的protocol吗?谢谢!
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Xiyan Li Author Answered Sep 16, 2011

Department of Genetics, Stanford University, USA

Since cell lysis is not involved in your experiment, you can use your sample as cell lysate in most assays developed for cells. Very general methods can also be found in literature or on the web, and commercial fluorescence-based reagents from several vendors such as Promega, Pierce, and others. Search for "beta-galactosidase fluorescence" will yield enough information.
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