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I have problms with the elution of the antibodies. I checked...

Yuanqing Lin
Yuanqing Lin Jul 12, 2011
protocol Protocol: Antibody Purification from Western Blotting
I have problms with the elution of the antibodies. I checked with a second antibody if those from the serum are still on the menbrane and they are still there. All of them. I don't get a signal on WB with the eluate, and BCA neither shows higher concentration of protein in comparison to el Read more Read less more less
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bio-protocol Answered Aug 31, 2011

"BCA neither shows higher concentration of protein in comparison to [elution buffer with Tris]. "

Did you elute with Glycine or Tris? Tris will not elute the protein at all. If you used Glycine, have you checked the pH which is critical for the elution.
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