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What the function of Potasium fero and feri cyanide in gus...

Yuanqing Lin
Yuanqing Lin Jul 8, 2011
protocol Protocol: Histostaining for Tissue Expression Pattern of Promoter-driven GUS Activity in Arabidopsis
What the function of Potasium fero and feri cyanide in gus substrate?
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bio-protocol Answered Jul 12, 2011

Ferro- and Ferri-cyanides form a system buffering redox status (mechanistically like pH buffer). The hydrolyzed indolyl half of X-Gluc by GUS needs to be oxidized to radical before it can form the dimerized blue precipitate as so-called GUS staining.
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