One question: In some protocols (very similar to yours), it's...

Yuanqing Lin
Yuanqing Lin
Jun 27, 2011
protocol Protocol: Labeling Protein with Thiol-reactive Probes
One question: In some protocols (very similar to yours), it's recommended as a final step (prior to separation on clumn) to add an excess of Gluthation or BetaMercaptoEthanol, to ensure no reactive species are present.
You do not recommended this step? Why?
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bio-protocol Answered Jul 10, 2011

Adding chemicals with thiol group, such as reduced glutathione and 2-Mercaptoethanol before the column separation of excess dye is optional. Gel filtration method can completely remove excess thiol-reactive reagent. The presence of reactive species have no effect on the label efficiency as well labeled protein.
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