I have prepared a frsh 10x PCR buffer as described and I had...

Yuanqing Lin
Yuanqing Lin
May 19, 2011
protocol Protocol: Standard PCR Protocol
I have prepared a frsh 10x PCR buffer as described and I had tested it. There was amplification however there is a trailing effect from the well.... So what could be the possible reason for this? And how can I trouble shoot this problem

chaitanya
scholar,
India
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Fanglian He Author Answered Aug 31, 2011

Bio-protocol

I am not sure about what you meant by "a trailing effect from the well".
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