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Assalamoalaikumi have a question regardind ligation reactionatually...

Yuanqing Lin
Yuanqing Lin May 14, 2011
protocol Protocol: Standard DNA Cloning
Assalamoalaikum
i have a question regardind ligation reaction
atually m facing problem with selfligation so i need a protocol for ligation of insert in vector by using alkaline phosphatase. how much vol. of this enzyme should be used in ligation reaction of final volume 30ul.
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Fanglian He Author Answered Aug 31, 2011

Bio-protocol

Treat vector with alkaline phosphatase before set up the ligation reaction. Please see this link http://www.bio-protocol.com/wenzhang.aspx?id=43&fl3=7 for protocol of using alkaline phosphatase.
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