what is the efficiency of transfection?

Step 3 has been corrected according to A1

Sorry, the iMAX dose for a well of a 6-well plate should be 4.8ul (1.2ul is for a well of a 24-well plate).

The efficiency is high, can usually reach 70%-90% knockdown in two-three days.

For some very highly-expressed targets, you may need to increase the siRNA concentration to 50nM-100nM and the iMAX dose to 7.5ul for a well of a 6-well plate or 1.5 ul for a well of a 24-well plate. Also, for some low-expressed targets, you can decrease the siRNA concentration to 20nM-30nM and the iMAX dose to to 3.6ul for a well of a 6-well plate or 0.9 ul for a well of a 24-well plate.