Purify small RNA fragments

jx
jun xiang
Mar 17, 2025
protocol Protocol: QsRNA-seq: A protocol for Generating Libraries for High-throughput Sequencing of Small RNAs

I'm glad to have read your team's experimental proposal. I have a question: Is it possible to purify small RNA fragments less than 50 nt using this method ?

best wishes!

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Alla Fishman Author Answered Mar 24, 2025

Of cause it is possible to separate fragments below 50nt. However, keep in mind that for efficient separation you need a difference of no less than 15nt between the fragments to be separated.

The optimal conditions for the separation depend on the size range of the fragments you wish to keep and of those you want to remove. Please refer to the table 1 of the paper which presents the binding efficiencies of ssDNA oligonucleotides to SPRI beads at variant Isopropanol concentrations. Optimal conditions for sizes not present in the table can be determined experimentally as described in the paper.


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