What are the most efficient methods for large-scale production of single-stranded DNA?

Dear Doctor, I have two questions to consult. 1. In the text, "The plasmid should have an antibiotic resistance gene other than Amp, Cmp and Kan" - does this mean it must have a different resistance gene? Would it be acceptable if it only has the ampicillin resistance gene? 2. What is t Read more Read less