How many cells to seed in 10cm plates for the assay?

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Sana Sharba
Mar 7, 2025
protocol Protocol: SUrface SEnsing of Translation (SUnSET), a Method Based on Western Blot Assessing Protein Synthesis Rates in vitro

For the assay, should we scale up the seeding density from 0.2 million in 6-well plate according to surface area to 0.55 million in the 10 cm plate.

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Cedric Chaveroux Author Answered Mar 10, 2025

CRCL Lyon

I recommend keeping the cell concentration appropriate for your experiments without necessarily adjusting the amount of puromycin. This is quite standard in our laboratory, and we keep the same final concentration of puromycin whatever the number of cells seeded.

Good luck.


C chaveroux

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