Hello, I would like to ask whether the sequence incorporated in the TMLC

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Shane Zhu
Feb 26, 2025
protocol Protocol: Measurement of Cell Intrinsic TGF-β Activation Mediated by the Integrin αvβ8

Hello, I would like to ask whether the sequence incorporated in the TMLC cells starts from position 1 of the first exon of PAI-1. I am currently quite confused about this issue. Thank you.

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Stephen Lloyd Nishimura Author Answered Mar 5, 2025

Univeristy of California San Francisco San Francisco

It's a truncated pai1 promoter construct. See the first reference. Abe, 1994, for a description.


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