What ultramicroscope settings can be used to achieve imaging of the entire depth of a rat brain?

Hi again,

I'd recommend calling the company, as the answer will depend on your exact microscope and parts available. We used a 1.1x objective with a 16mm working distance and the provided STL file for a brain holder/chamber. The dipping cap should be chosen based on the refractive index solution used and doesn't have an affect on the working distance. Note that udisco and idisco have different amounts of shrinkage of the brain which will also affect working distances required