Clarification on BODIPY Staining Solution Preparation and Final Concentration

Thank you for your question. To prepare the stock solution, follow the supplier's instructions to make a 1 mg/mL solution in DMSO. This stock solution can be stored at -20°C for up to one year. I recommend aliquoting it into smaller volumes (e.g., 1 mL) to avoid repeated freeze-thaw cycles.

For the working solution, dilute the stock solution further with DMSO to achieve a 1:25 dilution as specified in the protocol. For example, to prepare 1 mL of working solution for the staining step, combine 40 µL of the stock solution with 960 µL of DMSO. I hope this addresses your question clearly.