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Whether collagenase type IV or II could be used in the colon tissue digestion?

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yiqian chen Nov 3, 2024
protocol Protocol: Induction and Analysis of Anti-CD40-induced Colitis in Mice
  1. Whether collagenase type IV or II could be also used in the digestion of the colon tissue? And what about the choose of collagenase for small intestine tissue digestion?
  2. The second question is for mouse ILC3, gate on live cells, whether the gating strategy could be simpli Read more Read less more less
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David Bauché Author Answered Nov 4, 2024

Merck & Co., Inc., MRL, South San Francisco, USA, USA,

Hi Yiqian Chen,

Thanks for your questions.

  1. We have never tested Collagenase, type II. We used collagenase type IV for both colon and small intestine.
  2. For mouse ILC3, gating on lin-CD127+RORgt+ is acceptable.


Best,

David

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