Can you cryoperserve after DSP fixation and quenching of tissue?

Dear Chang,

Yes, indeed this is the approach used for long-term storage. We collect the tissue, mince it, and fix it to cryopreserve. When needed, we thaw the tissue, dissociate it, and proceed with the single-cell experiment

This protocol has been validated and documented in a benchmarking study, successfully applied to lung and gut tissues from both mouse and human samples in healthy and disease contexts. You can refer to the study for further insights on the methodology and its results: https://doi.org/10.1186/s13059-024-03219-5.

I hope that answered your question.

Best,