According to the Fig.1, after homogenisation, centrifuge 5 min, 600 g first, then save TH sample and keep SN for next step.
So, where is the TH sample from? Before centrifugation or after cuntrifugation (supernatent) ?
Another question is How many protease inhibit
Read moreMaría Isabel Hernández-Alvarez Author Answered Sep 18, 2024
University of Barcelona Barcelona
Dear Manxi Zhao,
The first step, 600 g 5 min, is only to collect the cells after the scraping. TH is collected only after the dounce homogenization, and it is directly from the mix that you obtain during this process without centrifuge.
Best,
Raúl Ventura Author Answered Sep 25, 2024
Regarding the second question related with how much protease inhibitors are we adding, we use a Protease Inhibitor Cocktail (PIC). It should be at x1 in every buffer.
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