If scale up DSP possible?

Hi,

The prep of DSP can’t be scaled without risking precipitation and suboptimal final concentration of DSP in the working solution.

We recommend to make 500 uL and pool aliquots.

Luciano

thanks for the reply, those are very helpful suggestion. We are doing some perturbation at large scale, so a higher volume is needed. I did test scale up in house, we found that if do it within 10 minutes and vigous vortexing, we can scale up to 10mL final solution without loss of efficiency.

Dear Jiang,

as we explained in this guide, DSP has very low solubility in PBS or most aqueous solutions, so that simply scaling up will form precipitation and therefore perturb the fixation efficiency. Depending on the exact amount of input, you may consider running test on your cells first. It is also possible to fix cells suspension in 500uL and replenish with additional aliquots for higher cell input, rather than generate a larger scale at once.

Could you provide some background information and please explain why you need multiple million cells? Regular single cell methods can't analyze that many cells at once.

Thank you! Let me know if that answered your question.