Some questions about RNA fragmentation

毅 段
Sep 11, 2024
protocol Protocol: Analysis of in vivo Interaction between RNA Binding Proteins and Their RNA Targets by UV Cross-linking and Immunoprecipitation (CLIP) Method

When doing RNA fragmentation, why do you want to experiment with the sample in Step C2, RNase I is not incubated at 37 °C but 4 °C, and the absence of cross-linking after UV cross-linking has any effect on the results

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