Improve Research Reproducibility A Bio-protocol resource

why can't the reverse primers match?

佳周 Aug 25, 2024

Protocol: Rapid Identification of Pathogens in Severe Pneumonia by Species-specific Bacterial Detector (SSBD)

Hello author, may I ask if I used NCBI to design primers for the first bacterium according to your method in this article, but why can't the reverse primers match? For the first bacterium, for example, what sequence should be entered at design time?

15 Views

Answer

Recommend

1 answer

Sort by: most helpful / most recent

Qihan Chen Author Answered Aug 26, 2024

Medical School of Nanjing University, Nanjing University, China

For A. baumannii, the PCR Template sequences can be referred to Table 5. The forward and reverse primers are matched to the 5' and 3' ends of the target sequences, respectively.

0 helpful

0 unhelpful

0 comments down

My answer

Write your answer...

References (optional)

add Add more

Post an Answer