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reagent for dissociating cells

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Ice Frost Moon Aug 13, 2024
protocol Protocol: Rapid and Simplified Induction of Neural Stem/Progenitor Cells (NSCs/NPCs) and Neurons from Human Induced Pluripotent Stem Cells (hiPSCs)

I see you choose Accutase, I wonder if I can use GCDR for replacement.
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Ice Frost Moon Answered Aug 22, 2024

Sichuan University

OK. Thanks for your reply.

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Tadahiro Numakawa Author Answered Aug 22, 2024

Department of Cell Modulation, Institute of Molecular Embryology and Genetics Kumamoto University

We have not tried GCDR for neural induction. Accutase is an enzyme-based reagent, while GCDR is enzyme-free. Therefore, we are not sure we can use GCDR for replacement.

We recommend you use Accutase in our protocol although you can give it a try!

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