In the very first step of makeblastdb, is one large database made with all the transcripts combined (eg. AALA translated nucleotides + AJJE translated nucleotides, ect) or are they all seperate blast databases to be searched against?
Sumanth Mutte Author Answered Aug 29, 2024
Laboratory of Biochemistry, Wageningen University, 2020-
Hello Jodie,
Sorry for my delayed response due to vacation period.
BLAST database was created for each transcriptome/proteome separately. So, each one is searched separately for the hits using BLAST.
Best regards,
Sumanth
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